The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis
Identifieur interne : 000784 ( Main/Exploration ); précédent : 000783; suivant : 000785The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis
Auteurs : Svetlana Chefer [États-Unis] ; Jurgen Seidel [États-Unis] ; Adam S. Cockrell [États-Unis] ; Boyd Yount [États-Unis] ; Jeffrey Solomon [États-Unis] ; Katie R. Hagen [États-Unis] ; David X. Liu [États-Unis] ; Louis M. Huzella [États-Unis] ; Mia R. Kumar [États-Unis] ; Elena Postnikova [États-Unis] ; J. Kyle Bohannon [États-Unis] ; Matthew G. Lackemeyer [États-Unis] ; Kurt Cooper [États-Unis] ; Ariel Endlich-Frazier [États-Unis] ; Heema Sharma [États-Unis] ; David Thomasson [États-Unis] ; Christopher Bartos [États-Unis] ; Philip J. Sayre [États-Unis] ; Amy Sims [États-Unis] ; Julie Dyall [États-Unis] ; Michael R. Holbrook [États-Unis] ; Peter B. Jahrling [États-Unis] ; Ralph S. Baric [États-Unis] ; Reed F. Johnson [États-Unis]Source :
- mSphere [ 2379-5042 ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Gènes rapporteurs, Infections à coronavirus (anatomopathologie), Instabilité du génome, Modèles animaux de maladie humaine, Mutagenèse par insertion, Souris transgéniques, Symporteurs (génétique), Symporteurs (métabolisme), Tomographie par émission monophotonique couplée à la tomodensitométrie ().
- MESH :
- anatomopathologie : Infections à coronavirus.
- croissance et développement : Coronavirus du syndrome respiratoire du Moyen-Orient.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Symporteurs.
- métabolisme : Symporteurs.
- Animaux, Cellules Vero, Gènes rapporteurs, Instabilité du génome, Modèles animaux de maladie humaine, Mutagenèse par insertion, Souris transgéniques, Tomographie par émission monophotonique couplée à la tomodensitométrie.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus Infections (pathology), Disease Models, Animal, Genes, Reporter, Genomic Instability, Mice, Transgenic, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (growth & development), Mutagenesis, Insertional, Single Photon Emission Computed Tomography Computed Tomography (methods), Symporters (genetics), Symporters (metabolism), Vero Cells.
- MESH :
- chemical , genetics : Symporters.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- growth & development : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : Symporters.
- methods : Single Photon Emission Computed Tomography Computed Tomography.
- pathology : Coronavirus Infections.
- Animals, Chlorocebus aethiops, Disease Models, Animal, Genes, Reporter, Genomic Instability, Mice, Transgenic, Mutagenesis, Insertional, Vero Cells.
Abstract
Advanced medical imaging such as single photon emission computed tomography with computed tomography (SPECT/CT) enhances fields such as oncology and cardiology. Application of SPECT/CT, magnetic resonance imaging, and positron emission tomography to infectious disease may enhance pathogenesis studies and provide alternate biomarkers of disease progression. The experiments described in this article focus on insertion of a SPECT/CT-compatible reporter gene into MERS-CoV to demonstrate that a functional SPECT/CT reporter gene can be inserted into a virus.
Url:
DOI: 10.1128/mSphere.00540-18
PubMed: 30541777
PubMed Central: 6291621
Affiliations:
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Le document en format XML
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Baric</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F." last="Johnson">Reed F. Johnson</name><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">30541777</idno><idno type="pmc">6291621</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291621</idno><idno type="RBID">PMC:6291621</idno><idno type="doi">10.1128/mSphere.00540-18</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">001253</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001253</idno><idno type="wicri:Area/Pmc/Curation">001253</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001253</idno><idno type="wicri:Area/Pmc/Checkpoint">000452</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000452</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:30541777</idno><idno type="wicri:Area/PubMed/Corpus">000709</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000709</idno><idno type="wicri:Area/PubMed/Curation">000709</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000709</idno><idno type="wicri:Area/PubMed/Checkpoint">000745</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000745</idno><idno type="wicri:Area/Ncbi/Merge">002038</idno><idno type="wicri:Area/Ncbi/Curation">002038</idno><idno type="wicri:Area/Ncbi/Checkpoint">002038</idno><idno type="wicri:Area/Main/Merge">000787</idno><idno type="wicri:Area/Main/Curation">000784</idno><idno type="wicri:Area/Main/Exploration">000784</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis</title><author><name sortKey="Chefer, Svetlana" sort="Chefer, Svetlana" uniqKey="Chefer S" first="Svetlana" last="Chefer">Svetlana Chefer</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Seidel, Jurgen" sort="Seidel, Jurgen" uniqKey="Seidel J" first="Jurgen" last="Seidel">Jurgen Seidel</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S." last="Cockrell">Adam S. Cockrell</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Solomon, Jeffrey" sort="Solomon, Jeffrey" uniqKey="Solomon J" first="Jeffrey" last="Solomon">Jeffrey Solomon</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Hagen, Katie R" sort="Hagen, Katie R" uniqKey="Hagen K" first="Katie R." last="Hagen">Katie R. Hagen</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Liu, David X" sort="Liu, David X" uniqKey="Liu D" first="David X." last="Liu">David X. Liu</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Huzella, Louis M" sort="Huzella, Louis M" uniqKey="Huzella L" first="Louis M." last="Huzella">Louis M. Huzella</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Kumar, Mia R" sort="Kumar, Mia R" uniqKey="Kumar M" first="Mia R." last="Kumar">Mia R. Kumar</name><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Postnikova, Elena" sort="Postnikova, Elena" uniqKey="Postnikova E" first="Elena" last="Postnikova">Elena Postnikova</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Bohannon, J Kyle" sort="Bohannon, J Kyle" uniqKey="Bohannon J" first="J. Kyle" last="Bohannon">J. Kyle Bohannon</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Lackemeyer, Matthew G" sort="Lackemeyer, Matthew G" uniqKey="Lackemeyer M" first="Matthew G." last="Lackemeyer">Matthew G. Lackemeyer</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Cooper, Kurt" sort="Cooper, Kurt" uniqKey="Cooper K" first="Kurt" last="Cooper">Kurt Cooper</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Endlich Frazier, Ariel" sort="Endlich Frazier, Ariel" uniqKey="Endlich Frazier A" first="Ariel" last="Endlich-Frazier">Ariel Endlich-Frazier</name><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Sharma, Heema" sort="Sharma, Heema" uniqKey="Sharma H" first="Heema" last="Sharma">Heema Sharma</name><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Thomasson, David" sort="Thomasson, David" uniqKey="Thomasson D" first="David" last="Thomasson">David Thomasson</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Bartos, Christopher" sort="Bartos, Christopher" uniqKey="Bartos C" first="Christopher" last="Bartos">Christopher Bartos</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Sayre, Philip J" sort="Sayre, Philip J" uniqKey="Sayre P" first="Philip J." last="Sayre">Philip J. Sayre</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Sims, Amy" sort="Sims, Amy" uniqKey="Sims A" first="Amy" last="Sims">Amy Sims</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Dyall, Julie" sort="Dyall, Julie" uniqKey="Dyall J" first="Julie" last="Dyall">Julie Dyall</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Holbrook, Michael R" sort="Holbrook, Michael R" uniqKey="Holbrook M" first="Michael R." last="Holbrook">Michael R. Holbrook</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B." last="Jahrling">Peter B. Jahrling</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F." last="Johnson">Reed F. Johnson</name><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">mSphere</title><idno type="eISSN">2379-5042</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (pathology)</term><term>Disease Models, Animal</term><term>Genes, Reporter</term><term>Genomic Instability</term><term>Mice, Transgenic</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (growth & development)</term><term>Mutagenesis, Insertional</term><term>Single Photon Emission Computed Tomography Computed Tomography (methods)</term><term>Symporters (genetics)</term><term>Symporters (metabolism)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Gènes rapporteurs</term><term>Infections à coronavirus (anatomopathologie)</term><term>Instabilité du génome</term><term>Modèles animaux de maladie humaine</term><term>Mutagenèse par insertion</term><term>Souris transgéniques</term><term>Symporteurs (génétique)</term><term>Symporteurs (métabolisme)</term><term>Tomographie par émission monophotonique couplée à la tomodensitométrie ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Symporters</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Symporteurs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Symporters</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Single Photon Emission Computed Tomography Computed Tomography</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Symporteurs</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Disease Models, Animal</term><term>Genes, Reporter</term><term>Genomic Instability</term><term>Mice, Transgenic</term><term>Mutagenesis, Insertional</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Gènes rapporteurs</term><term>Instabilité du génome</term><term>Modèles animaux de maladie humaine</term><term>Mutagenèse par insertion</term><term>Souris transgéniques</term><term>Tomographie par émission monophotonique couplée à la tomodensitométrie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Advanced medical imaging such as single photon emission computed tomography with computed tomography (SPECT/CT) enhances fields such as oncology and cardiology. Application of SPECT/CT, magnetic resonance imaging, and positron emission tomography to infectious disease may enhance pathogenesis studies and provide alternate biomarkers of disease progression. The experiments described in this article focus on insertion of a SPECT/CT-compatible reporter gene into MERS-CoV to demonstrate that a functional SPECT/CT reporter gene can be inserted into a virus.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hoenen, T" uniqKey="Hoenen T">T Hoenen</name></author><author><name sortKey="Groseth, A" uniqKey="Groseth A">A Groseth</name></author><author><name sortKey="Callison, J" uniqKey="Callison J">J Callison</name></author><author><name sortKey="Takada, A" uniqKey="Takada A">A Takada</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marsh, Ga" uniqKey="Marsh G">GA Marsh</name></author><author><name sortKey="Virtue, Er" uniqKey="Virtue E">ER Virtue</name></author><author><name sortKey="Smith, I" uniqKey="Smith I">I Smith</name></author><author><name sortKey="Todd, S" 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Jahrling</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Chefer, Svetlana" sort="Chefer, Svetlana" uniqKey="Chefer S" first="Svetlana" last="Chefer">Svetlana Chefer</name></region><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S." last="Baric">Ralph S. Baric</name><name sortKey="Bartos, Christopher" sort="Bartos, Christopher" uniqKey="Bartos C" first="Christopher" last="Bartos">Christopher Bartos</name><name sortKey="Bohannon, J Kyle" sort="Bohannon, J Kyle" uniqKey="Bohannon J" first="J. Kyle" last="Bohannon">J. Kyle Bohannon</name><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S." last="Cockrell">Adam S. Cockrell</name><name sortKey="Cooper, Kurt" sort="Cooper, Kurt" uniqKey="Cooper K" first="Kurt" last="Cooper">Kurt Cooper</name><name sortKey="Dyall, Julie" sort="Dyall, Julie" uniqKey="Dyall J" first="Julie" last="Dyall">Julie Dyall</name><name sortKey="Endlich Frazier, Ariel" sort="Endlich Frazier, Ariel" uniqKey="Endlich Frazier A" first="Ariel" last="Endlich-Frazier">Ariel Endlich-Frazier</name><name sortKey="Hagen, Katie R" sort="Hagen, Katie R" uniqKey="Hagen K" first="Katie R." last="Hagen">Katie R. Hagen</name><name sortKey="Holbrook, Michael R" sort="Holbrook, Michael R" uniqKey="Holbrook M" first="Michael R." last="Holbrook">Michael R. Holbrook</name><name sortKey="Huzella, Louis M" sort="Huzella, Louis M" uniqKey="Huzella L" first="Louis M." last="Huzella">Louis M. Huzella</name><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B." last="Jahrling">Peter B. Jahrling</name><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B." last="Jahrling">Peter B. Jahrling</name><name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F." last="Johnson">Reed F. Johnson</name><name sortKey="Kumar, Mia R" sort="Kumar, Mia R" uniqKey="Kumar M" first="Mia R." last="Kumar">Mia R. Kumar</name><name sortKey="Lackemeyer, Matthew G" sort="Lackemeyer, Matthew G" uniqKey="Lackemeyer M" first="Matthew G." last="Lackemeyer">Matthew G. Lackemeyer</name><name sortKey="Liu, David X" sort="Liu, David X" uniqKey="Liu D" first="David X." last="Liu">David X. Liu</name><name sortKey="Postnikova, Elena" sort="Postnikova, Elena" uniqKey="Postnikova E" first="Elena" last="Postnikova">Elena Postnikova</name><name sortKey="Sayre, Philip J" sort="Sayre, Philip J" uniqKey="Sayre P" first="Philip J." last="Sayre">Philip J. Sayre</name><name sortKey="Seidel, Jurgen" sort="Seidel, Jurgen" uniqKey="Seidel J" first="Jurgen" last="Seidel">Jurgen Seidel</name><name sortKey="Sharma, Heema" sort="Sharma, Heema" uniqKey="Sharma H" first="Heema" last="Sharma">Heema Sharma</name><name sortKey="Sims, Amy" sort="Sims, Amy" uniqKey="Sims A" first="Amy" last="Sims">Amy Sims</name><name sortKey="Solomon, Jeffrey" sort="Solomon, Jeffrey" uniqKey="Solomon J" first="Jeffrey" last="Solomon">Jeffrey Solomon</name><name sortKey="Thomasson, David" sort="Thomasson, David" uniqKey="Thomasson D" first="David" last="Thomasson">David Thomasson</name><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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